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24/7 Onsite

DOT Drug Testing

Federal DOT-regulated drug and alcohol testing in full compliance with 49 CFR Part 40.

DOT drug testing is mandated by the Department of Transportation for safety-sensitive employees in the transportation industry. All testing must comply with 49 CFR Part 40 regulations. 24/7 Onsite ensures every collection meets these strict federal standards.

Types of DOT Testing

  • Pre-employment testing
  • Random testing
  • Post-accident testing
  • Reasonable suspicion testing
  • Return-to-duty testing
  • Follow-up testing

DOT Agencies We Serve

  • Federal Motor Carrier Safety Administration (FMCSA)
  • Federal Aviation Administration (FAA)
  • Federal Railroad Administration (FRA)
  • Federal Transit Administration (FTA)
  • Pipeline and Hazardous Materials Safety Administration (PHMSA)
  • United States Coast Guard (USCG)

Our collectors follow consistent protocols to assure proper handling of all collections. Every specimen is collected according to DOT procedures with full chain of custody documentation.